Barbora studied international business at the Prague University of Economics and Business. She worked in the economic department of the Czech Embassy in Rome when she started to provide expert advisory services to health and lifestyle companies entering the Italian market. During her nine years of experience in international business, she also managed clients in North and South America, Asia, and Australia for several companies. For the last two years, she has been developing the B2B business of GymBeam, the fastest-growing active and healthy lifestyle brand in the CEE region.

He graduated from the University of Brno (1983–1988) with a degree in Czech language and history, after which he worked as a historian in a museum. In the nineteen eighties he was involved in independent civic activities against the communist dictatorship. After November 1989 he founded the field of political science. The positions he has held since then include head of the Department of Political Science (1993–2002) and the Department of International Relations and European Studies (2002–2004), director of the International Institute of Political Science (1996–2004), director of the Institute for Comparative Political Research (2005–2011) and the dean of the Faculty of Social Studies at Masaryk University (2004).

As rector he served two terms as head of Masaryk University (2004–2011), which during that time became the most popular Czech university and one of Central Europe’s leading educational and research institutions. For many years he has worked for institutions and bodies focusing on university education and research in the Czech Republic and abroad, and was also the chair of the Czech Rectors’ Conference (2009–2011). He has held a number of public and professional offices, including, for example, membership of the Board of the Institute for the Study of Totalitarian Regimes, the Accreditation Commission, the vice-chairmanship of the Research, Development and Innovation Council, and served as the chief scientific adviser to the Prime Minister. In his scientific work he specialises in comparative political science, European politics and conservative political thinking. He is the author of more than 20 books and 300 professional studies published in the Czech Republic and abroad.

In 2012–2013 he was Minister of Education in Petr Nečas’s government; he succeeded in bringing stability to the department and his conceptual and convincing work quickly won him the trust of the general public.

In October 2013 he was a non-partisan candidate for the South Moravian ODS and was elected as a member of parliament. He joined ODS on 7 November 2013, and was elected party leader in January 2014. He retained his leadership in January 2016, 2018 and 2020. In October 2017 he was the ODS nationwide and South Moravian leader in the elections, in which ODS became the second strongest political party. In November of the same year he was elected Deputy Speaker of the Chamber of Deputies of the Parliament of the Czech Republic. In autumn 2020 he became the nationwide leader of the newly formed electoral coalition SPOLU (ODS, KDU-ČSL, TOP 09). In October 2021 the SPOLU coalition won the parliamentary elections with a percentage of 27.8 %. He was appointed Prime Minister on 28 November 2021. Members of his Government were appointed on 17 December 2021.